Fig. 5. Kidney formation in Cp2l1^tra/tra mice. (A,B) PAS staining of a kidney section at postnatal day 2. Higher magnification figures of A are shown in B. (C) Expression of AQP2 protein in the medulla was normal in both newborn Cp2l1^+/tra and Cp2l1^tra/tra mice. (D) Expression of Pax2 in CD (arrowheads) was normal even in Cp2l1^tra/tra mice at birth. (E) Quantification of mRNA expression levels of nephron segmental marker genes at birth by quantitative RT-PCR. Values represent mean expression levels ± standard deviation (n=4-9). The experiments were repeated at least twice, and there was no significant difference between Cp2l1^+/tra and Cp2l1^tra/tra mice. Scale bars: 200 µm.