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Figure 6


Fig. 6. HAP2 protein is sperm-localized during pollen development and tube growth. (A) A pollen tetrad and (B) sperm nuclei from a plant heterozygous for the HAP2protein:YFP fusion; DAPI fluorescence (left), YFP fluorescence (center), merged image (right). (C) DAPI (left) and YFP (right) fluorescence in pollen tetrads from homozygous HAP2protein:YFP transgenic plants at three stages of pollen development; see Fig. 5D for comparison with qrt. (D) DAPI (center) and YFP (right) fluorescence in growing pollen tubes from qrt, HAP2protein:YFP and LAT52:GFP transgenic plants. DIC images of growing pollen tubes (left). Signal from YFP is observed only in the sperm (HAP2protein:YFP) or throughout the entire pollen tube cytoplasm (LAT52:GFP). Scale bars: 20 µm A and C; 1 µm in B; 5 µm in C.