Fig. 2. Cell death in L/Ser-mutant cells is p53-independent and caspase-dependent. (A-B') Overexpression of p53 (A) in the wild-type eye using ey-GAL4 (ey>p53) results in a small eye and, in the L²/+-mutant eye (L²/+; ey>p53; B,B'), does not affect the loss-of-ventral-eye phenotype. Dashed line marks the lost boundary of the eye field. (C-D') Blocking caspase-dependent cell death by overexpression of baculovirus P35 in the wild-type eye (C) does not affect eye size whereas, in the L²/+-mutant background (L²/+; ey>p53), this can significantly rescue the loss-of-ventral-eye phenotype in the eye imaginal disc (D) and adult eye (D'). (E-H') Overexpression of diap1 (ey>diap1; E) or a reduction in the levels of the Hid-Reaper-Grim complex by using deficiency H99 (G) in wild-type eye does not affect eye size, whereas, in L²/+-mutant backgrounds (F,F',H,H'), these can rescue the loss-of-ventral-eye phenotype. (I) Overexpression of dominant-negative Ser (ey>Ser^DN) results in loss of ventral eye (Singh and Choi, 2003). Dashed line marks the lost boundary of the eye field. (J,J') Blocking caspase-dependent cell death can significantly rescue the loss-of-ventral-eye phenotype of dominant-negative Ser overexpression (ey>Ser^DN+P35), as seen in eye disc (J) and adult eye (J'). Markers for immunostaining are shown in color labels.