Fig. 2. Ontogeny of p63 on sagittal sections of wild-type embryos (fluorescent immunohistochemistry, 100x). Sagittal sections of E11.5 embryos transect the epithelium tangentially at the distal UGS, accounting for the wider expression pattern at the distal urogenital sinus (A,E). (A-D) p63 (4A4) expression. (E-H) Np63 isoform expression. (I-L) TAp63 isoform expression. Blood cells within the mesenchyme are autofluorescent. (M) RT-PCR of wild-type E15.5 bladder, using adenoviruses with Np63, TAp63, p63, p63ß and p63 constructs as controls. Arrows in A-J and M represent the positive immunoreactivities and RT-PCR bands of wild-type bladder samples. B, wild-type bladder cDNA; V, adenoviruses containing Np63, TAp63, p63, p63ß and p63 constructs.