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Figure 5


Fig. 5. (A,B) Hematoxylin and Eosin staining of the sagittal sections of E11.5 wild-type and p63-/- embryos (100x). (C,D) Fluorescent TUNEL staining (arrows) of wild-type and p63-/- UGS (200x). The DAPI staining of nuclei is shown in red to increase color contrast. (E,F) The colorimetric immunostaining (arrows) for cleaved caspase-3 in E11.5 wild-type and p63-/- UGS (200x). (G,H) Fluorescent TUNEL staining (arrows) of sagittal sections through the oral cavity of E11.5 wild-type and p63-/- embryos (400x). (I,J) The qPCR relative expressions of Bax and Apaf1 in E12.5 and E13.5 p63-/- bladders. (A,C,E,G) Wild type. (B,D,F,H) p63-/-. HG, hindgut.