Fig. 2. Spatial distribution of apoptosis among migratory germ cells. (A,B) Cleaved-PARP immunostaining (red) labels apoptotic cells, including PGCs (white arrows) and the mesonephroi (arrowheads), which are known to degenerate during this time period (Pole et al., 2002). (A) A transverse section showing the direction of imaging (blue arrows) of whole-mount embryos in B. (B) Apoptotic PGCs (yellow) occurred in greater numbers in the midline (area between white lines). (C) Quantification of PGC apoptosis in B, showing enhanced death amongst midline PGCs (n=8). Scale bars: 100 µm.