Fig. 3. Germ cell expression of apoptosis-related genes. (A) Real-time RT-PCR shows that pro-apoptotic genes, including Bcl2 superfamily members Bax, Bak, Bad and Bim, were downregulated in postmigratory PGCs. (B) Genes with gender-specific differential regulation include Fas and Bcl-x, which increased in postmigratory germ cells in the male, and Casp6, which was upregulated in the female from E13.5-14.5. E10.5 was considered baseline for statistical comparisons, except for Bcl-x, for which E11.5 was considered baseline. F, female; M, male. ^**P<0.001.