Fig. 5. Slice culture experiments. (A,C) Germ cells in E10.5 embryo slices survive in the midline in the presence of Steel factor in a dose-dependent manner. Midline PGCs scored as ectopic in (C) are marked with arrows in A. No significant change in total PGC numbers was observed in the different treatment groups in A. (B,D) Conversely, PGCs cultured with Ack2 died in a dose-dependent manner. Error bars represent means ± s.e.m. (n=8-12).