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Figure 7


Fig. 7. Bax is required for E9.0 germ cell death in the absence of Steel. (A-D) Immunostaining of Bax/Steel E9.0 embryos for cleaved PARP shows that apoptosis occurs at a higher frequency in Steel-/- embryos, and is reduced by the absence of Bax. (A) Bax+/- Steel+/-, (B) Bax-/- Steel+/-, (C) Bax+/- Steel-/-, (D) Bax-/- Steel-/-. Scale bars: 100 µm. (E) Germ cell numbers were significantly reduced in Bax+/* Steel-/- embryos compared with Bax+/* Steel+/* littermates (* denotes + or -), but are rescued by the loss of Bax. (F) The percentage of cleaved-Parp+ PGCs is significantly increased in Bax+/* Steel-/- embryos compared with Bax+/* Steel+/* littermates, but is rescued by the loss of Bax. {ddagger}P<0.05, {ddagger}{ddagger}P<0.01. Error bars represent means ± s.e.m.