Fig. 3. Predicted topology and deduced amino acid sequence of Snky. (A) Predicted features of Snky are shown with six transmembrane (TM) domains (blue cylinders) as predicted by the SOSUI program (Hirokawa et al., 1998), three highly conserved regions a-c (red bars), a predicted coiled-coil domain (orange) and conserved Cys residues (yellow circles). A C4-C4 RING finger is located near the carboxyl terminus. Asterisks (^*) indicate location of residues altered in snky^Z mutants. (B) Deduced amino acid sequence showing features diagrammed above. TM domains (boxed blue), highly conserved regions a-c (red), coiled-coil domain (underlined) and conserved Cys residues (C, boxed yellow).