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Figure 5


Fig. 5. Elimination of spliced Tsix RNA does not affect the methylation of the Xist promoter region. CpG methylation in the 5 ' region of Xist was analyzed in E13.5 embryos by Southern blotting. Diagrams of the promoter region of Xist show relevant restriction enzyme sites and the position of the probe used for this assay. CpG methylation was appropriately established even in the absence of spliced Tsix RNA in both sexes. B, BclI; H, HhaI; S, SacII.