Fig. 2. Ndst1-mutant ocular phenotypes. (A) The range of ocular phenotypes observed in E12.5 to E17.5 Ndst1^KO/KO embryos. (B-I) E13.5 Ndst1 embryos and eye sections showing Pax6 RNA in situ staining. Severe lens-developmental defects were observed in E13.5 Ndst1-mutant embryos (D-I), ranging from small or absent lenses to a complete lack of eyes. (J-S) Retinal patterning in the Ndst1 mutant. RNA in situ hybridization was performed on E14.5 embryos. Sox2, Six3, Hes1, Math5 and BF2 were expressed in both wild-type and Ndst1^KO/KO retinae. Notice the lack of lenses in Ndst1^KO/KO eyes. KO/KO, homozygous Ndst1-knockout embryos; L, lens.