Fig. 6. Disruption of heparan sulfate synthesis in Ndst1 mutants. (A) Specificities of Hepss-1, 10E4 and 3G10 heparan sulfate antibodies. (B) Loss of sulfation of heparin sulfate in KO/KO embryos. Hepss-1 and 10E4 antibodies were specific for heparan sulfate. Their staining in the developing lens was lost both in heparitinase I-treated (+ Heparitinase) wild-type embryos and in Ndst1 mutants. By contrast, staining by 3G10 antibody detected the heparan sulfate stub region after heparitinase I cleavage. Staining was observed in both wild-type and mutant embryos. KO/KO, homozygous Ndst1-knockout embryos.