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Figure 6


Fig. 6. Migration defects in Tbx20 mutants are not due to developmental arrest. (A-D) Flat-mounted hindbrains from E11.5 SE1::gfp mice before (A) and after (B-D) organotypic culture. Facial neurons migrate caudally after 30 hours in vitro (compare white brackets in A and B). (E-H) Isl1 expression in flat-mounted hindbrain explants from E11.5 Tbx20 mutants or controls. Facial neurons from Tbx20 mutants do not migrate, in contrast to the controls. Asterisk in F marks abducens SM neurons at r5. Trigeminal neurons from Tbx20 mutants fail to migrate laterally; compare medial (yellow arrowhead) and lateral (red arrowhead) Isl1+ cells in Tbx20 mutants and controls. Scale bars: in F, 250 µm for A-F; in H, 320 µm for G,H.