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Figure 7


Fig. 7. A genetic interaction between Tbx20 and Tbx2. (A-F) In situ hybridization analysis on flat-mounted hindbrain preparations and transverse sections reveals that Tbx2 expression is absent in Tbx20 mutants. The {Delta}Tbx20 probe detects both full-length and mutated transcripts. Adjacent sections were used to locate Tbx20-null cells. (G-I) Mis-expression of Phox2a induces Isl1, Tbx20 and Tbx2 expression in chick embryonic spinal cord at HH stage 20. Scale bars: in D, 400 µm for A,D; in F, 250 µm for B,C,E,F; in I, 100 µm for G-I.