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Figure 3


Fig. 3. Tlr is a circulatory enzyme. Western blots showing Tlr and Tlr(HA)3 (A) or ß-Gal (B). The serum protein serves as a loading control. The immunoblots were simultaneously probed for non-tagged/endogeneous Tlr (anti-Tlr) and tagged/overexpressed Tlr(HA)3 (12CA5) or mouse anti-ß-Gal. S2, transfected S2 cells; H, hemolymph from third-instar larvae of the indicated genotype; C, third-instar carcasses; *, non-specific bands.