Fig. 1. Molecular characterization of daw. (A) Genomic organization of the daw region. The synaptotagmin (syt) gene is located 5.5 kb centromere distal of daw and CG2964 is located 300 bp proximal of daw. Alternate isoforms daw-A and daw-B, which share common coding exons, are shown. The P{EP}EP1039 insertion site, genomic rescue fragment Daw-XmnI and deletions daw¹ and daw² are indicated. (B) Sequence and conceptual translation of daw (Genbank accession no. AY051485). Italics indicate signal sequence, boxes are proteolytic cleavage sites, and circles mark cysteine residues conserved in TGF-ß and activin ligands. Lesions in daw³ (deletion of CA) and daw⁴ (GT substitution) are highlighted in gray. (C) Clustal-W alignment of ligand domains of human TGF-ß3, activinC, BMP3, and Drosophila TGF-ß superfamily members. Identities are in dark gray, similarities in light gray, and asterisks mark conserved cysteines.