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Figure 6


Fig. 6 . Synaptic nuclei are not transcriptionally specialized in Lrp4 mutant muscles. E14.5 (A-H) or E18.5 (I-P) diaphragms showing in situ hybridizations using probes specific to AChR{alpha} (A,E,I,M) AChR{delta} (B,F,J,N), Musk (C,G,K,O) and Lrp4 (D,H,L,P). At E14.5, transcripts of these genes become restricted to the endplate band region in wild-type muscles (A-D) but are more broadly expressed in Lrp4 mutants (E-H). At E18.5,transcripts of these genes continue to be upregulated in the endplate band region in wild-type muscles (I-L) but are expressed at low, uniform levels in mutants (M-P).