Fig. 6. Short-range activity of Hh-N in columnar cells. dpp-lacZ (red) expression in wild-type wing imaginal disc (A) or around clones of cells (marked by GFP in green) expressing either Hh-Np (B-D), Hh-N (E-G) or Hh-N-CD2 (H-J) produced in columnar cells using the flip-out technique. (D,G,J) Confocal z-sections (apical side upwards). Clones of cells expressing Hh-Np in the anterior compartment of the disc induced dpp expression in a six- or seven-cell wide region around the clone (B-D), while Hh-N was able only to activate dpp expression in a three- or four-cell region around the clone, even when the clones were bigger than Hh-Np expressing clones (E-G). Hh-N-CD2 expressing clones activated dpp in 1 row of cells around the clone (H-J).