Fig. 1. The promoter of the ecmA gene. A representation of the ecmA promoter showing the positions of the regions known to direct pstA and pstO specific expression. The minimal region that directs pstO-specific expression is a 132 bp subfragment, located just over 1 kb upstream of the ecmA cap site (Kawata et al., 1996). The structures of the various mutants mentioned in the text are displayed below (see also Fig. 6B legend). The symbol Act15 indicates the basal promoter elements of the actin 15 gene (Pears et al., 1988). The figure also shows the positions and sequences of R1 and R2, the regulatory elements analysed in the present study.