Fig. 13. ChIP analysis of DimB. Ax2 cells or dimB- cells were induced in the manner indicated, and their chromatin was incubated with or without anti-DimB (-DimB) or with anti-CudA antibody (-cudA). PCR was performed on the immunoprecipitated DNA and the data were analysed as described in the text. The asterisk indicates the sample against which all other fluorescence intensities were normalized. The ecmA primers used (see Materials and methods) derive from upstream of R2 but the extent of sonication is such that the R2 region will register in the PCR. In addition, two other primer combinations, which derive from sequences more proximal to R2 were used, in other experiments and they also showed enrichment.