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Fig. 7. Developmental time course of DimB accumulation. Ax2 cells were allowed to develop to the indicated stages and harvested: t6=streaming; t10~11=tight aggregate; t12~13=tipped aggregate/first finger; t14-16=slug; t20=mid culminant. Western transfer was performed with the C terminus-specific DimB antibody and with an antibody to GSK3: a gene that is semi-constitutively expressed during development.