Fig. 8 Genetic analysis of DimB function. (A) Isolation of dimB-mutants. A mixture of putative dimB disruptants (marked with an asterisk) and random integrants (i.e. clones where the disruption vector integrated at a position other than the dimB locus) was analysed by western transfer with the C terminus-specific DimB antibody. The lane on the left contains DimB:HIS fusion protein and those on the right were loaded with Ax2 total protein isolated at the slug stage. (B) Morphological and behavioural phenotypes of the dimB- mutant. (Top) A dimB- and a control (random integrant) strain were developed on water agar to the slug stage. (Bottom) A dimB- and a control (random integrant) strain were developed on water agar in a darkened chamber with a narrow slit for 48 hours. The slugs were then lifted, with their adherent trails, onto a clear plastic sheet which was stained with Coomassie Blue.