Fig. 2. Developmental regulation and cell type expression of dimB RNA. Cells were developed on agar for the times indicated (hours) and RNA was harvested. After reverse transcription, levels of each transcript were determined by quantitative PCR and normalized to IG7. (A) Developmental regulation of dimB compared with dimA. Expression of the two genes is upregulated during development. (B) RNA from prespore and prestalk cells was analyzed by quantitative RT-PCR with oligonucleotides specific to the dimB mRNA, indicating expression in both cell types with some prespore enrichment. Primers against prestalk (ecmA) and prespore (cotB) specific genes were used as controls. Expression levels are given in arbitrary units.