Fig. 7. DIF-1-dependent nuclear accumulation of DimA and DimB. DimA and DimB rapidly accumulate in the nucleus of DIF-1-treated cells. DimA and DimB were constitutively expressed as GFP fusion proteins in cells starved for 4 hours (A,B) or 10 hours (C,D). After DIF-1 treatment, the number of cells with even staining in cytoplasm and nucleus rapidly decreased with a concomitant increase in the number of cells with stronger staining in the nucleus compared with cytoplasm. No change in distribution was observed in untreated control cells. Nuclear accumulation was verified by comparison with DAPI staining. In the pictures, 10 minutes DIF-1 treatment is shown. In the samples starved for 10 hours (C,D), clumping of cells can be seen. In addition, an increase in the number of cells with increased nuclear fluorescence is observed in control samples, presumably owing to endogenous DIF-1 production at this time point.