Fig. 8. Quantification of the nuclear accumulation kinetics. (A,B) DimA and DimB exhibit rapid nuclear accumulation kinetics. Cells with higher levels of nuclear fluorescence than cytoplasmic fluorescence were scored as positive. (C) Nuclear accumulation of DimA-GFP is dependent on DimB. DimB-GFP was expressed in dimA^- cells and DimA-GFP was expressed in dimB^- cells. Nuclear accumulation of DimB-GFP was unaffected, whereas no DIF-1-induced nuclear accumulation of DimA-GFP could be detected.