Fig. 5. Radial glia cells continue to accumulate in Ngn2 mutant mice, but the number of Nurr1⁺ cells partially recovers at E14.5. (A-D) By E13.5, Hes5 (A,B) and Dll1 (C,D) expression are significantly recovered in zone 1 VZ. (E,F) Cresyl violet staining highlights the enlarged ventricular zone in Ngn2 null mice at E14.5. (G-J) This cyto-architectural change is mirrored by the accumulation of radial glia cells, marked by RC2 /GLAST (G,H) or Sox2/GLAST (I,J) double staining. (K-M) At E14.5, the reduction in the number of Nurr1⁺ cells in Ngn2 mutants was less pronounced than at E11.5 (see Fig. 4G), indicating a partial rescue of the block in differentiation of DA neuron precursors (K, n=3; ^**P<0.01, Student's t-test with Welch's correction). Scale bar: 100 µm.