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Fig. 4. Loss of medially located mesDA neuron precursors in the Ngn2 mutant. (A,D,G,J,M,P) At all stages of mesDA neurogenesis (E11.5-E15.5), Nurr1 staining showed a lack of Nurr1-positive mesDA neuron precursors in the medial region of the VM in Ngn2 mutants. (E,K,Q) Interestingly, GFP expressed from the Ngn2 locus revealed the presence of cells in the medial region of the Ngn2 mutants. (B,E,H,K,O,S) GFP staining; (C,F,I,L,O,R) merged images. Scale bars: 100 µm.