Fig. 6. Diffuse expression of Reln in control and hem-ablated mice. (A-C) Sagittal sections through E15.5 brains; (D-F) coronal sections through E18.5 neocortex. At E15.5 and E18.5 in control mice (A,D), but not hem-ablated mice (B,E), a dense layer of Reln+ CR cells fills the MZ (asterisk in B indicates the region of hem loss and consequent failure of hippocampal development). Below this is more diffuse Reln expression. At E15.5, a band of low Reln+ expression appears in the IZ/SVZ in both mutants and controls (A-C). By E18.5, scattered Reln+ cells appear in layers IV/V (D-F). (F) Layers defined by expression of Rorb: Reln+ cells are yellow, Rorb expression is light purple. Arrows in each panel indicate Reln+ cells.