Fig. 2. Spatiotemporal expression pattern of Fz5/8 during embryogenesis. (A) Temporal analysis by northern blotting with total RNA isolated from embryos at indicated stages. (B) Spatial distribution of Fz5/8 transcripts revealed by whole-mount in situ hybridization. All embryos were fixed at indicated stages and hybridized with Fz5/8 antisense probe, except for double labeling (where Fz5/8 probe was used simultaneously with ske-T or Brachyury probes). Lateral views with the animal pole at the top. E, unfertilized egg; 16, 16-cell stage; 60, 60-cell stage; B1-5, blastula stages; HB, hatched blastula; MB, mesenchyme blastula; EG, early gastrula; G, gastrula; Pr, prism; P, pluteus.