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Fig. 5. Polarity of FzTM1-injected larvae. (A) DIC (left) and dark-field (right) images of FzTM1-injected larvae. (B) Determination of the morphological polarity of FzTM1-injected larvae by labeling one of the two blastomeres at the two-cell stage. (C) Confocal images of neurons immunostaining on FzTM1-injected larvae. Red, 1e11 antibody (Nakajima et al., 2004); green, counterstain. Control embryo, animal pole view (left); FzTM1-injected embryos, oral view (middle) and transverse view (right).