Fig. 7. Requirement of Fz5/8 signaling in SMC for archenteron and skeleton formation. (A) Experimental design. At the 16-cell stage, animal and vegetal halves of FzTM1-injected embryos (red) were isolated and transplanted to the complementary half of a normal embryo (white). (B) Experimental results. B1-B3, control glycerol-injected embryo; B4-B6, control embryo injected with FzTM1 and a red lineage tracer; B7-B9, injected animal half recombined with control vegetal half; B10-B12, injected vegetal half transplanted with control animal half. (B1,B4,B7,B10) DIC images of resulting larvae. (B2,B5,B8,B11) Epifluorescence images of the same embryos, showing the contribution of the transplanted halves. (B3,B6,B9,B12) Confocal images of the larvae stained with EndoI (endoderm antibody) in red (Wessel and McClay, 1985) and with 1d5 (PMC marker) in green (Hardin et al., 1992).