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Figure 6


Fig. 6. Cl- removal triggers rapid pHi increases in oocytectomised (OOX) cumulus-oocyte complexes. (A) Examples of OOX complexes produced by aspirating the oocyte from within cumulus-oocyte complexes (see Materials and methods). (B) pHi was recorded during removal of Cl- (at 10 minutes) in SNARF-AM loaded complexes. Cl- removal caused a very rapid increase in pHi (0.91±0.02 pH units/minute). Data shown is the average of three replicates, from a total of 11 complexes. Inset shows an example of fluorescence images of OOX complexes.