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Figure 4


Fig. 4. The inhibition of germline proliferation in daf-2 mutants largely requires daf-16 activity. L1 animals were grown at 25°C for the specified times, and the total number of germ cells was determined as described in the Materials and methods. Germline proliferation was also examined in daf-16(mgDf50); daf-2(e1370) animals (data not shown), and the results obtained were similar to daf-16(mgDf47); daf-2(e1370). Error bars indicate s.d. Sample size was >10 for each point.