Fig. 2. uri-1(lf) mutants have defects in germ cell proliferation. (A) Number of germ nuclei per gonad arm in uri-1(RNAi)F1 (n=25) and wild-type (n=25) worms. Progeny of staged animals were synchronized by letting the P0 generation to lay eggs for 2 hours, fixed at the indicated time points and stained with DAPI. Germ cells identified by their DNA morphology were counted. Error bars represent s.d. (B) Images of gld-2(If) gld-1(If) (top) and gld-2(If) gld-1(If) uri-1(RNAi)F1 (bottom) gonads. Loss of URI-1 results in reduction of mitotic germ cells. For both images, anterior is towards the left and dorsal towards the top. Scale bar: 10 µm.