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Figure 4


Fig. 4. Accumulation of DNA breaks in the adult hermaphrodite germline due to loss of uri-1 function. (A) (Top) Histograms depicting quantification of TUNEL foci in uri-1(RNAi)F1 (n=10) and wild-type (n=10) gonads at 25°C. The nuclei within a 15 nuclei diameter radius from the distal tip cell were scored. Each column represents the percentage of nuclei that contain a defined number of foci. (Bottom) TUNEL staining in wild-type and uri-1 germlines is shown. Anterior is towards the left and dorsal is towards the top. (B) Quantification (left) of the HUS-1::GFP foci in uri-1(RNAi)F1 (gray) and control RNAi (black) in hus-1(op241); unc-119(ed3); opIs34 mitotic germ cells (n=55 for each, *P=3.4x10-43) and image of germ nuclei (right) showing the relocalization of HUS-1::GFP. OpIS34 is a HUS-1::GFP integrated line. Error bars represent s.d. Scale bar: 5 µm.