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Figure 8


Fig. 8. Phospho-MAPK immunohistochemistry. Whole-mount phospho-MAPK immunohistochemistry of stage-matched embryos: (A-C) E8.5, (D-F) E9.5. High magnification views of (B,E) caudal gastrulating regions and (C,F) more rostral areas undergoing somitogenesis and neurulation. Arrowheads and arrows indicate phospho-MAPK-positive somites and neural folds, respectively. (G-L) G/J, H/K and I/L are stage-matched embryo pairs (E10.5). (G,H,J,K) Pharyngeal arch 1, 2 region; (I) dorsal and (L) lateral views of premigratory neural crest cells (arrows). Lateral view is shown in L because this embryo has an open neural tube.