Fig. 11. Inhibition of FAK alters Xena localization. (A) Xena is enriched at the cortex in control animal caps. (B) Inhibition of FAK results in decrease in the levels of Xena at the cortex. (C) Plot of pixel intensity in control animal caps showing enrichment of Xena at the cortex. (D) Plot of pixel intensity in FRNK-injected animal caps reveals that Xena is de-localized from the cortex and displays a more uniform distribution. (E) Quantitative analysis of Xena staining in control (GFP) and FRNK injected BCRs. For 10 randomly selected cells, pixel intensity was measured along a line extending across the region of cell-cell contact. Average pixel intensity at the membrane (two peak intensities 0.3 µm apart) was compared with the average pixel intensity of the juxtamembrane region (3.3 µm adjacent to membrane). The ratio of the average membrane intensity to average juxtamembrane intensity was significantly lower for FRNK-expressing embryos. P<0.001 (Student's t-test). Error bars indicate s.d. n=50 cells per treatment.