Fig. 7. Inhibition of Ena/VASP leads to disruption of the FN matrix. (A-D) Stacked z-series of FN staining reveals a fragmentation of the matrix (arrowheads), separation of somites from overlying ectoderm (arrows), gaps in the intersomitic FN matrix (asterisks) and loss of defined boundaries between the somite and neural tube or notochord in FP₄-mito (bracket) (A), but not AP₄-mito (C) injected embryos. Injected side is on the right. High magnification images of the boundary between the dorsal somite and neural tube reveals a disruption in the integrity of the FN matrix in FP₄-mito (B) but not AP₄-mito (D) injected embryos. (E-G) Stacked z-series show a dense FN matrix (white) on the interior BCR surface of an uninjected embryo (green, E) and an embryo injected with AP₄-mito (green, F). By contrast, expression of FP₄-mito (G) blocks FN matrix assembly as revealed by a thin or absent FN matrix in areas of GFP signal. s, somite; nt, neural tube. Scale bars: 100 µm.