Fig. 2. WEE-1.3 depletion causes precocious oocyte maturation. (A-F) Wild-type (A,C,E) and WEE-1.3-depleted (B,D,F) animals stained with pH3 antibodies (A,B), NOP1 antibodies (C,D), and TOTO-3 (E,F). Scale bar: 20 µm. (G) Quantitation of pH3- and NOP1-positive nuclei by oocyte position in the proximal gonad. Below each graph is a table showing the number of oocytes analyzed. (H) Quantitation of the presence of an intact NE in the -1 oocyte from adult hermaphrodites untreated (WT) or depleted of WEE-1.3 or WEE-1.1 (control). Animals were analyzed 16-20 hours post-injection. n, number of gonad arms analyzed.