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Figure 5


Fig. 5. Microtubules organisation in control and char RNAi embryos. (A) Nuclear localisation during cellularisation after injection of colcemid to depolymerise microtubules (MTs). Neurotactin (green) marks the plasma membrane, Even-skipped (blue) labels the nuclei. Arrows indicate misaligned nuclei. (B-H) MTs organisation in control (B,D,F) and char RNAi (C,E,E',G,H) embryos, during early (B,C) and late (F-H) cellularisation. MTs ({alpha}-tubulin) are in green, nuclei (Hoechst) are in blue and the membrane invagination front (Patj) is in red. Arrowheads show apical astral microtubules. The white arrow indicates a falling nucleus in char RNAi embryo in H. (D-E') Grazing sections showing astral MTs in the control (D) and char RNAi (E,E') embryos. Section planes (z1-z3) are schematised in I. (E'') Detail of a grazing section at z3 of the bottom nucleus shown in H.