Fig. 7. Char localises to the nuclear envelope. (A,B) Char antibody staining (green) highlights the nuclear envelope during cellularisation in a control embryo (A) but is absent in a char RNAi embryo (B). (C-F) Char staining in embryos from heterozygous flies for a deficiency covering the char locus (Dfchar), viewed from the top (C,D, insets) and in sagittal sections (E,F). Two categories of embryos, inferred to be, respectively, homozygous DfChar embryos and heterozygous siblings, are observed: in the first, the nuclei have a normal morphology and high levels of Char at the NE (C,E); in the second, the nuclei display the distinct charRNAi nuclear envelope phenotype together with a low expression of Char (D,F). Scale bars: 5 µm.