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Figure 1


Fig. 1. Regulation of EGF family expression by O2. (A) Immunohistochemical labeling of EGF family members in cytotrophoblast cells. Antibodies against EGF, TGF{alpha} (TGF-a), HBEGF, amphiregulin (AR), betacellulin (BC) and epiregulin (ER) were used, as well as a non-immune goat IgG control. (B) Antibody labeling was quantified by image analysis in cells cultured for 24 hours at either ambient O2 levels (black bars) or 2% O2 (gray bars). *P<0.05; **P<0.001, compared with ambient culture.