Fig. 6. Conservation of SBE4 sequence and function. (A) Vista plots comparing the alignment of human SBE4 sequences with mouse, chicken, frog and tetraodon. (B) Comparison of the distance between SBE4 and the Shh transcription start site in human, mouse, chicken, frog and tetraodon. (C-E) SBE4 reporter activity derived from (C) chicken, (D) frog and (E) pufferfish sequences. The ratio of embryos exhibiting reproducible Shh-like reporter activity over the total number of transgenic embryos is indicated for each construct (C-E).