Fig. 2. Immunostaining of DKO mouse lens with anti-active caspase 3 antibodies. Both primary anti-active caspase 3 antibody and secondary anti-rabbit IgG antibodies, conjugated with Alexa Fluor 488, were used for detection (A). The bright-field image was captured (B). As a negative control, the primary antibody was omitted, and only the secondary antibody was used (C). The bright field image (B) and active caspase 3 fluorescent image (A) were merged for morphological comparison (D). Scale bar: 250 µm; A-C are at the same magnification.