Fig. 7. Western blots of cobalt chelate gel column elution fractions.
Recombinant caspase 6 (0.6 µg, left lane), 30 µl of the elution fraction
from the control column, where caspase 6 was omitted (middle lane), and 30
µl of the elution fraction from the caspase 6-Co2+ column (right
lane) were separated on a 10% NuPage gel and detected by western blot with
anti-caspase 6, anti-
B-crystallin or anti-
A-crystallin
antibodies.