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Figure 4


Fig. 4. Combined loss of Slit2 and Ntn1 leads to abnormal EB morphology with characteristics of both Slit2-/-and Ntn1-/- EBs. All EBs immunostained with antibody generated against SMA. (A-C) Slit2-/-;Ntn1-/- EBs. (A) Loss of LECs underlying the cap cell layer (between arrowheads). (B) Separation of the cap cell layer from the LEC layer (double arrowhead) and breaks in the basal lamina (rectangles). (C) Complete disruption of EB morphology characterized by the infolding of cap cells into the LEC compartment (arrowhead) leading to lumen loss. (D) +/+ EB. L, lumen. Scale bar: 20 µm.