Fig. 5. Expression of netrin 1 and Dcc in the developing telencephalon. (A-H) Expression of netrin 1 (A-D) and Dcc (E-H) mRNAs in coronal sections of E10.5 (A,E), E11.5 (B,F) and E12.5 (C,G) wild-type and E12.5 Xt^J/Xt^J telencephalons (D,H). Asterisks indicate the presumptive LOT area. In wild-type embryos, netrin 1 is strongly expressed in the neuroepithelium of the GE during all these embryonic stages and weakly on the surface of the olfactory tubercle at E12.5 (arrowhead in C). Dcc is expressed in the surface layer of the neocortex. The expression patterns are essentially similar in the Xt^J/Xt^J telencephalon. (I-M) Double-immunostaining of a coronal section of E12.5 telencephalon (I-L) and cultured cortical neurons (M) with mAb lot1 (white in K; magenta in I,J,M) and anti-Dcc antibody (white in L; green in I,J,M). (J-L) are enlargements of the boxed area in I. The cortical neurons in M were dissociated from the E11.0 neocortex and cultured for 36 hours before the immunostaining as described previously (Tozaki et al., 2002). All lot1-positive neurons express Dcc. Scale bars: 500 µm in A-I; 20 µm in J,M.