Fig. 4. Cadherin-mediated adhesion in ß-catenin CKO embryos. (A) Double immunolabeling of ß-catenin (ß-cat) and E-cadherin (E-cad), -catenin (-cat) or -catenin (-cat) at E8.75 and E9.0. Brackets indicate ß-catenin-negative regions of ectoderm in CKO embryos. (B) Double immunolabeling of E10.5 embryos with ß-catenin and either E-cadherin or -catenin. Dashed ovals indicate the outline of ß-catenin-negative otocysts. Both E-cadherin and -catenin are expressed in the ß-catenin-negative cysts. (C) Neurogenesis in ß-catenin CKO embryos at E10.5. A Neurod1-positive ganglion can be seen delaminated from the small CKO otocyst (arrowhead). This ganglion is a mixture of ß-catenin-positive (red, white arrow) and negative (dashed line) cells. ß-Catenin-negative cells are still ßIII tubulin (TuJ1, green) positive. t, trigeminal; g, geniculate; cv, cochleovestibular; p, petrosal and n, nodose ganglia. Scale bars: 100 µm.