Fig. 1. Morphology of Notch1 CKO retinae. (A,B) Hematoxylin and Eosin staining of retinae from 3-week old wild-type (A) and Notch1 CKO (B) mice. The white arrowheads indicate rosette-like structures. (C) Schematic illustration of NIN and NIN-CRE constructs. Representative two- and one-cell clones from NIN infection. (D) Quantitation of clone sizes resulting from NIN or NIN-CRE infections on Notch1 flox/flox retinae. Two independent retinae totaling 300-350 clones were analyzed for each type of virus. Change in clone size in the NIN-CRE-infected retinae compared with control (NIN-infected retinae) is statistically significant (^*P<0.05, two-tailed t-test assuming equal variances). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, outer segment.